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Review
. 2017 Jul-Sep;33(3):199-206.
doi: 10.4103/iju.IJU_9_17.

Laboratory tests for oxidative stress

Ashok Agarwal  1 , Ahmad Majzoub  2
Affiliations
Review

Laboratory tests for oxidative stress

Ashok Agarwal et al. Indian J Urol. 2017 Jul-Sep.

Abstract

Introduction: Oxidative stress (OS) is considered a significant contributor to male infertility. A number of laboratory techniques have been developed to evaluate oxidative stress in the semen. We review these tests and their current use.

Methods: A literature review was performed utilizing the PubMed search engine for articles studying OS etiology and impact on male fertility, and the laboratory tests used in its assessment.

Results: The state of OS results from exaggerated production of oxygen-derived free radicals, also known as reactive oxygen species, to an extent overwhelming the body's antioxidant defense mechanisms. Several laboratory tests have been utilized in OS measurement during male fertility evaluation. These tests are classified into direct assays which measure the degree of oxidation within a sperm cell and indirect assays which estimate the detrimental effects of OS. The chemiluminescence assay, flow cytometry, nitroblue tetrazolium assay, and cytochrome c reduction are examples of direct assays while the myeloperoxidase test and measurements of lipid peroxidation, oxidation-reduction potential, and total antioxidant capacity are examples of the indirect assays.

Conclusion: OS measurement is an important tool that may help in understanding the pathophysiology of male infertility and provide valuable information that would guide treatment decisions and patient follow-up.

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Conflict of interest statement

Conflicts of interest: There are no conflicts of interest.

Figures

Figure 1
Figure 1
Etiology of oxidative stress
Figure 2
Figure 2
Reactive oxygen species measurement by chemiluminescence assay: AutoLumat 953 Plus Luminometer connected to a computer with a sample result graph
Figure 3
Figure 3
The MiOXSYS system comprises an (a) analyzer, (b) a disposable sensor, (c) application of the sample
See this image and copyright information in PMC

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