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. 2017 Jun;54(7):2135-2144.
doi: 10.1007/s13197-017-2653-1. Epub 2017 Apr 28.

Determination of anthocyanins and non-anthocyanin polyphenols by ultra performance liquid chromatography/electrospray ionization mass spectrometry (UPLC/ESI-MS) in jussara (Euterpe edulis) extracts

Gláucia S Vieira  1 , Anna S F Marques  2 , Mariana T C Machado  1 , Vanessa M Silva  1 , Miriam D Hubinger  1
Affiliations

Determination of anthocyanins and non-anthocyanin polyphenols by ultra performance liquid chromatography/electrospray ionization mass spectrometry (UPLC/ESI-MS) in jussara (Euterpe edulis) extracts

Gláucia S Vieira et al. J Food Sci Technol. 2017 Jun.

Abstract

This work aimed to propose two analytical methods for the quantitative and qualitative analysis of major anthocyanins and non-anthocyanin phenolic compounds in jussara (Euterpe edulis) extracts, using ultra performance liquid chromatography-mass spectrometry. These methods were evaluated for selectivity, precision, linearity, detection and quantification limits. The complete separation of 5 anthocyanins and 22 non-anthocyanins polyphenols was achieved in 4.5 and 7 min, respectively. Limits of detection ranged from 0.55 to 9.24 µg/L, with relative standard deviation for concentration up to 7.0%. In jussara extract, 13 of the 27 analytes were characterized. The dominant compound was cyanidin-3-O-rutinoside, representing about 73% of the total phenolic compounds content (approximately 23 mg/g of extract in dry weight). Other phenolic compounds found in the extract were: cyanidin-3-O-glucoside, pelargonidin-3-O-glucoside, quercetin, rutin, myricetin, kaempferol, kaempferol-3-O-rutinoside, luteolin, apigenin, catechin, ellagic acid and 4,5-dicaffeoylquinic acid.

Keywords: Color; Cyanidin-3-rutinoside; Flavonoid; Keracyanin; Oenin; Red pigments.

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Conflict of interest statement

The authors declare that there is no conflict of interest

Figures

Fig. 1
Fig. 1
Overlayed total ion UPLC/MS chromatograms of mixture of the standards. Anthocyanins (a): dephinidin-3-O-glucoside (1), cyanidin-3-O-glucoside (2), cyanidin-3-O-rutinoside (3), perlagonidin-3-O-glucoside (4), malvinidin-3-O-glucoside (5). Non-anthocyanic phenolic compounds (b): gallic acid (1), protocatechuic acid (2), p-hydroxybenzoic acid (3), catechin (4), chlorogenic acid (5), caffeic acid (6), p-coumaric acid (7), ferrulic acid (8), sinapic acid (9), hydroxycinamic acid (10), ellagic acid (11), rutin (12), kaempferol-3-O-rutinoside (13), kaempferol-3-O-glucoside (14), 4,5-dicaffeoylquinic acid (15), myricetin (16), resveratrol (17), luteolin (18), quercetin (19), apigenin (20), kaempferol (21), crisin (22)
Fig. 2
Fig. 2
Selected ion recording (SIR) chromatograms of the anthocyanins found in jussara extract, detected in positive ionization mode (ES+): cyanidin-3-O-glucoside (1), cyanidin-3-O-rutinoside (2) and perlagonidin-3-O-glucoside (3)
Fig. 3
Fig. 3
Selected ion recording (SIR) chromatograms of non-anthocyanins polyphenolics found in jussara extract, detected in negative ionization mode (ES−): catechin (1), ellagic acid (2), rutin (3), kaempferol-3-rutinoside (4), 4,5-dicaffeoylquinic acid (5), myricetin (6), luteolin (7), quercetin (8), apigenin (9), kaempferol (10)
See this image and copyright information in PMC

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