Emergence of different Acinetobacter baumannii clones in a Croatian hospital and correlation with antibiotic susceptibility

Abstract

Objectives: During routine diagnostic laboratory work, the clinical microbiologist observed an increase of Acinetobacter baumannii isolates with three different carbapenem susceptibility patterns: susceptible, intermediate and resistant. Isolates belonging to the same carbapenem susceptibility phenotype exhibited identical susceptibility/resistance patterns to non-β-lactam antibiotics. This prompted us to analyse the mechanisms of carbapenem-resistance and the molecular epidemiology of the isolates. A total of 59 A. baumannii isolates were analysed and grouped according to their susceptibility to imipenem: group 1 were susceptible (N=24), group 2 were intermediate (N=8) and group 3 were resistant (N=27) to imipenem.

Material and methods: PCR and sequencing was used to detect resistance genes. Genotyping of the isolates was performed by PFGE and MLST.

Results: Out of 27 resistant isolates, 20 harboured bla_OXA-40-like and 7 bla_OXA-23-like genes. ISAba1 was found upstream of bla_OXA-51 and bla_OXA-23 genes. PFGE genotyping demonstrated the existence of three major A. baumannii clones in GH Pula and determination of sequence groups showed that the isolates belonged to international clones commonly associated with multidrug-resistance. MLST (performed on six isolates) showed diverse population structure of isolates belonging to the same cluster, including ST 195, ST 231, ST 775 and ST 1095.

Conclusions: A previous study conducted in 2009-2010 showed that reduced susceptibility to carbapenems in GH Pula was only associated with upregulation of the intrinsic OXA-51 β-lactamase. In this study a shift to isolates with acquired oxacillinases, belonging to two major clones was reported.

Keywords: Acinetobacter baumannii; Carbapenem-hydrolyzing oxacillinase; Carbapenems; Resistance.