Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Jul;52(1):197-9.
doi: 10.1128/aem.52.1.197-199.1986.

Modified plasmid isolation method for Clostridium perfringens and Clostridium absonum

I Roberts, W M Holmes, P B Hylemon

Modified plasmid isolation method for Clostridium perfringens and Clostridium absonum

I Roberts et al. Appl Environ Microbiol. 1986 Jul.

Abstract

A rapid plasmid isolation procedure for Clostridium perfringens and C. absonum is described. The ratio of culture volume to lysis buffer volume was found to be crucial for efficient plasmid isolation. The method can be scaled up, without difficulty, for large-scale plasmid preparation.

PubMed Disclaimer

References

    1. J Bacteriol. 1973 Nov;116(2):1064-6 - PubMed
    1. Anal Biochem. 1976 Dec;76(2):431-41 - PubMed
    1. Antimicrob Agents Chemother. 1978 May;13(5):871-80 - PubMed
    1. Appl Environ Microbiol. 1984 Jul;48(1):178-81 - PubMed
    1. J Bacteriol. 1981 Jul;147(1):262-6 - PubMed

Publication types

MeSH terms

LinkOut - more resources