Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Dec;199(10):1383-1389.
doi: 10.1007/s00203-017-1412-z. Epub 2017 Jul 24.

Detection of Verticillium species in Swedish soils using real-time PCR

Georgios Tzelepis  1 , Sarosh Bejai  2 , Muhammad Naeem Sattar  2   3 , Arne Schwelm  2 , Jonas Ilbäck  2   4 , Johan Fogelqvist  2 , Christina Dixelius  2
Affiliations

Detection of Verticillium species in Swedish soils using real-time PCR

Georgios Tzelepis et al. Arch Microbiol. 2017 Dec.

Abstract

Verticillium species are soilborne plant pathogens, responsible for big yield losses worldwide. Here, we report improved procedures to generate DNA from Verticillium species imbedded in farm soils. Using new genomic sequence information, primers for V. dahliae, V. albo-atrum, V. tricorpus, and V. longisporum were designed. In a survey of 429 samples from intensively farmed soil of two Swedish regions, only V. dahliae and V. longisporum were identified. A bias towards V. longisporum (40%) was seen in the south, whereas V. dahliae was more frequent in the western region (19%). Analyses of soil and leaf samples from 20 sugar beet fields, where foliar wilting had been observed, revealed V. dahliae DNA in all leaf and soil samples and V. longisporum in 18 soil samples, illustrating host choice and longevity of the V. longisporum microsclerotia. This study demonstrates the applicability of new molecular diagnostic tools that are important for growers of variable crops.

Keywords: Beta vulgaris; Brassica napus; Soilborne pathogens; Verticillium; qPCR.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Analysis of Verticillium primers specificity. Primers listed in Table 2 of a V. longisporum, b V. dahliae, c V. albo-atrum, d V. tricorpus were tested against the other Verticillium species. Amplification was observed only on-target Verticillium species. No amplification on off-target species and on mock samples was observed. Melt curve analysis shows amplification of a single fragment. Two technical replicates were used
See this image and copyright information in PMC

References

    1. Almquist C, Persson L, Olsson Å, Sundström J, Jonsson A. Disease risk assessment of sugar beet root rot using quantitative real-time PCR analysis of Aphanomyces cochioides in natural infested soil samples. Eur J Plant Pathol. 2016;145:731–742. doi: 10.1007/s10658-016-0862-5. - DOI
    1. Andersson C (2003) Detection methods of Verticillium longisporum in soil and in oilseed rape. M.Sc. Dissertation 45: ISSN 1651-5196, Swedish University of Agricultural Sciences (SLU)
    1. Atallah ZK, Bae J, Jansky SH, Rouse DI, Stevenson WR. Multiplex real-time quantitative PCR to detect and quantify Verticillium dahliae colonization in potato lines that differ in response to Verticillium wilt. Phytopathology. 2007;97:865–872. doi: 10.1094/PHYTO-97-7-0865. - DOI - PubMed
    1. Chaouachi M, Alaya A, Ali IB, Ben Hafsa A, Nabi N, Berard A, Romaniuk M, Skhiri F, Said K. Development of real-time PCR method for the detection and the quantification of a new endogenous reference gene in sugar beet “Beta vulgaris L.”: GMO application. Plant Cell Rep. 2013;32:117–128. doi: 10.1007/s00299-012-1346-5. - DOI - PubMed
    1. Debode J, van Poucke K, Franca SC, Maes M, Hofte M, Heungens K. Detection of multiple Verticillium species in soil using density flotation and Real-Time Polymerase Chain reaction. Plant Dis. 2011;95:1571–1580. doi: 10.1094/PDIS-04-11-0267. - DOI - PubMed

LinkOut - more resources