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Review
. 2017 Jul 11:4:45.
doi: 10.3389/fmolb.2017.00045. eCollection 2017.

Probing Long Non-coding RNA-Protein Interactions

Jasmine Barra  1   2 , Eleonora Leucci  1
Affiliations
Review

Probing Long Non-coding RNA-Protein Interactions

Jasmine Barra et al. Front Mol Biosci. .

Abstract

Non-coding RNA sequences outnumber the protein-coding genes in the human genome, however our knowledge of their functions is still limited. RNA-binding proteins follow the transcripts, including non-coding RNAs, throughout their life, regulating not only maturation, nuclear export, stability and eventually translation, but also RNA functions. Therefore, development of sophisticated methods to study RNA-protein interactions are key to the systematic characterization of lncRNAs. Although mostly applicable to RNA-protein interactions in general, many approaches, especially the computational ones, need adjustment to be adapted to the length and complexity of lncRNA transcripts. Here we critically review all the wet lab and computational methods to study lncRNA-protein interactions and their potential to clarify the dark side of the genome.

Keywords: RBPs; RNA; RNA immunoprecipitation; crosslinking; pull-down assays.

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Figures

Figure 1
Figure 1
Workflow for the identification of RNA-protein interactions.
See this image and copyright information in PMC

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