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. 2017 Dec;107(12):1496-1506.
doi: 10.1094/PHYTO-12-16-0444-R. Epub 2017 Sep 29.

Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes

Meriem Aoun  1 , James A Kolmer  1 , Matthew N Rouse  1 , Shiaoman Chao  1 , Worku Denbel Bulbula  1 , Elias M Elias  1 , Maricelis Acevedo  1
Affiliations

Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes

Meriem Aoun et al. Phytopathology. 2017 Dec.

Abstract

Leaf rust, caused by Puccinia triticina, and stem rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of leaf rust resistance (Lr) genes to P. triticina race BBBQJ and stem rust resistance (Sr) genes to P. graminis f. sp. tritici race TTKSK in durum accessions. Eight leaf-rust-resistant genotypes were used to develop biparental populations. Accessions PI 192051 and PI 534304 were also resistant to P. graminis f. sp. tritici race TTKSK. The resulting progenies were phenotyped for leaf rust and stem rust response at seedling stage. The Lr and Sr genes were mapped in five populations using single-nucleotide polymorphisms and bulked segregant analysis. Five leaf-rust-resistant genotypes carried single dominant Lr genes whereas, in the remaining accessions, there was deviation from the expected segregation ratio of a single dominant Lr gene. Seven genotypes carried Lr genes different from those previously characterized in durum. The single dominant Lr genes in PI 209274, PI 244061, PI387263, and PI 313096 were mapped to chromosome arms 6BS, 2BS, 6BL, and 6BS, respectively. The Sr gene in PI 534304 mapped to 6AL and is most likely Sr13, while the Sr gene in PI 192051 could be uncharacterized in durum.

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Figures

Fig. 1
Fig. 1
Distance in centimorgans (cM) between simple sequence repeat (SSR) and kompetitive allele-specific polymerase chain reaction (KASP) assay single-nucleotide polymorphism markers linked to the leaf rust (Puccinia triticina race BBBQJ) resistance gene (LrPI209274) on chromosome arm 6BS using phenotypic and genotypic data of the recombinant inbred lines of the cross Rusty × PI 209274 at F6 generation.
Fig. 2
Fig. 2
Distance in centimorgans (cM) between kompetitive allele-specific polymerase chain reaction (KASP) assay single-nucleotide polymorphism markers linked to the leaf rust (Puccinia triticina race BBBQJ) resistance gene (LrPI387263) on chromosome arm 6BL using phenotypic and genotypic data of the recombinant inbred lines of the cross Rusty × PI 387263 at F6 generation.
Fig. 3
Fig. 3
Distance in centimorgans (cM) between kompetitive allele-specific polymerase chain reaction (KASP) assay single-nucleotide polymorphism markers linked to the leaf rust (Puccinia triticina race BBBQJ) resistance gene (Lr244061) on chromosome arm 2BS using phenotypic and genotypic data of F2 plants of the cross Divide × PI 244061.
See this image and copyright information in PMC

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