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. 2017 Jul 26;6(7):e67.
doi: 10.1038/emi.2017.52.

A two-step algorithm for rapid diagnosis of active pulmonary tuberculosis in entry applicants using the T-SPOT.TB and Xpert MTB/RIF assays in Shanghai, China

Chengyan Meng  1 , Yaojie Shen  2   3 , Jian Wang  1 , Sen Wang  2 , Xinchang Chen  2 , Shenglei Yu  2 , Wei Ye  1 , Jing Wu  2
Affiliations

A two-step algorithm for rapid diagnosis of active pulmonary tuberculosis in entry applicants using the T-SPOT.TB and Xpert MTB/RIF assays in Shanghai, China

Chengyan Meng et al. Emerg Microbes Infect. .
No abstract available

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Figures

Figure 1
Figure 1
The diagnostic flowchart. Of the 68 entry applicants, 23 were sputum culture-positive for MTB and were confirmed as having TB; 45 individuals were sputum culture-negative for MTB and were confirmed as not having TB. A two-step algorithm using T-SPOT followed by Xpert could be used for the rapid diagnosis of TB. Using T-SPOT as Step 1, 47 of 68 applicants were T-SPOT positive and 21 of 68 applicants were T-SPOT-negative. Using Xpert as Step 2, 14 of 68 applicants were Xpert-positive and 52 of 68 applicants were Xpert-negative, with two ‘invalid’ results. For the rapid diagnosis of TB, T-SPOT can be used as a rule-out test followed by Xpert as a rule-in test. T-SPOT-positive, Xpert-negative patients need to be followed up with repeat chest radiography, sputum smear and culture tests every 1–2 months. Orange: individuals with positive results for the indicated test; Green: individuals with negative results for the indicated test.
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