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. 2017 Sep;111(6):289-296.
doi: 10.1080/20477724.2017.1356052. Epub 2017 Jul 26.

The role of midgut symbiotic bacteria in resistance of Anopheles stephensi (Diptera: Culicidae) to organophosphate insecticides

Aboozar Soltani  1 , Hassan Vatandoost  2 , Mohammad Ali Oshaghi  2 , Ahmad Ali Enayati  3 , Ali Reza Chavshin  4
Affiliations

The role of midgut symbiotic bacteria in resistance of Anopheles stephensi (Diptera: Culicidae) to organophosphate insecticides

Aboozar Soltani et al. Pathog Glob Health. 2017 Sep.

Abstract

In the current study, the effects of the presence of symbiotic bacteria on the activity of the enzymes involved in An. stephensi resistance to temephos are evaluated for the first time. Four different strains (I. susceptible strain, II. resistant strain, III. resistant strain + antibiotic, and IV. resistant strain + bacteria) were considered in order to determine the possible effects of the symbiotic bacteria on their hosts' resistance to temephos. The median values of all enzymes of susceptible strain were compared with those of other resistant strains. The results of this study indicated a direct relationship between the presence of bacteria in the symbiotic organs of An. stephensi and resistance to temephos. The profile of enzymatic activities in the resistant strain changed to a susceptible status after adding antibiotic. The resistance of An. stephensi to temephos could be completely broken artificially by removing their bacterial symbionts in a resistant population.

Keywords: Anopheles stephensi; Insecticide resistance; Symbiotic bacteria; Temephos.

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Figures

Figure 1.
Figure 1.
Activity profile of all tested enzymes (a: α-esterase enzyme, b: ß-esterase enzyme, c: PNPA-esterase enzyme, d: MFO enzyme, e: GST enzyme, f: Percentage of the remaining activity of AChE enzyme) in different strains under study.
See this image and copyright information in PMC

References

    1. Oshaghi MA, Yaghoobi F, Vatandoost H, et al. . Anopheles stephensi biological forms, geographical distribution, and malaria transmission in malarious regions in Iran. Pak J Biol Sci. 2006;9:294–298.
    1. Hanafi-Bojd AA, Vatandoost H, Oshaghi MA, et al. . Entomological and epidemiological attributes for malaria transmission and implementation of vector control in southern Iran. Acta Trop. 2012 Feb 29;121:85–92.10.1016/j.actatropica.2011.04.017 - DOI - PubMed
    1. Vatandoost H, Shahi H, Abai MR, et al. . Larval habitats of main malaria vectors in Hormozgan province and their susceptibility to different larvicides. Southeast Asian J Trop Med Public Health. 2004;35:22–25. - PubMed
    1. Demaio J, Pumpuni CB, Kent M, et al. . The midgut bacterial flora of wild Aedes triseriatus, Culex pipiens, and Psorophora columbiae mosquitoes. Am J Trop Med Hyg. 1996 Feb;54:219–223.10.4269/ajtmh.1996.54.219 - DOI - PubMed
    1. Touré AM, Mackey AJ, Wang ZX, et al. . Bactericidal effects of sugar-fed antibiotics on resident midgut bacteria of newly emerged anopheline mosquitoes (Diptera: Culicidae). J Med Entomol. 2000 Mar 1;37:246–249.10.1093/jmedent/37.2.246 - DOI - PubMed

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