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. 2017 Jun;33(2):150-156.
doi: 10.5625/lar.2017.33.2.150. Epub 2017 Jun 30.

Comparison of the response using ICR mice derived from three different sources to multiple low-dose streptozotocin-induced diabetes mellitus

Do Yeon Lee  1 , Myeong Hwan Kim  1 , Hye Rin Suh  1 , Young Suk Jung  2 , Dae Youn Hwang  3 , Kil Soo Kim  1
Affiliations

Comparison of the response using ICR mice derived from three different sources to multiple low-dose streptozotocin-induced diabetes mellitus

Do Yeon Lee et al. Lab Anim Res. 2017 Jun.

Abstract

This study was conducted to compare the multiple low-dose streptozotocin (MLDS)-induced diabetic patterns of Korl:ICR, A:ICR, and B:ICR mice obtained from three different sources. Six-week-old male ICR mice were obtained from three difference sources. Korl:ICR mice were kindly provided by the National Institute of Food and Drug Safety Evaluation (NIFDS). The other two groups of ICR mice were purchased from different vendors located in the United States (A:ICR) and Japan (B:ICR). All ICR mice that received MLDS exhibited overt diabetic symptoms throughout the study, and the incidence and development of diabetes mellitus were similar among the three ICR groups. The diabetic mice exhibited hyperglycemia, loss of body weight gain, decreased plasma insulin levels, impaired glucose tolerance, decreased number of insulin-positive cells, and decreased size of β-cells in the pancreas. The diabetes symptoms increased as the blood glucose level increased in the three ICR groups. In particular, the level of blood glucose in the STZ-treated group was higher in Korl:ICR and A:ICR mice than in B:ICR mice. The plasma insulin levels, glucose tolerance, blood chemistry, and morphological appearance of the pancreas were very similar in the ICR groups obtained from the three different sources. In conclusion, our results suggest that Korl:ICR, A:ICR, and B:ICR mice from different sources had similar overall responses to multiple low-dose STZ to induce diabetes mellitus.

Keywords: Korl:ICR mice; diabetes mellitus; hyperglycemia; multiple low-dose streptozotocin.

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Conflict of interest statement

Conflict of interests: The authors declare that there is no financial conflict of interests to publish these results.

Figures

Figure 1
Figure 1. The body weight in multiple low dose streptozotocin induced diabetic ICR mice. The data shown represent the means±SD (n=7 per group). a, P<0.05 significant difference vs. Korl:ICR group. b, P<0.05 significant difference vs. A:ICR group. c, P<0.05 significant difference vs. B:ICR group.
Figure 2
Figure 2. The blood glucose levels in multiple low dose streptozotocin induced diabetic ICR mice. The data shown represent the means±SD (n=7 per group). a, P<0.05 significant difference vs. Korl:ICR group. b, P<0.05 significant difference vs. A:ICR group. c, P<0.05 significant difference vs. B:ICR group.
Figure 3
Figure 3. The plasma insulin levels in multiple low dose streptozotocin induced diabetic ICR mice. The data shown represent the means±SD (n=7 per group). a, P<0.05 significant difference vs. Korl:ICR group. b, P<0.05 significant difference vs. A:ICR group. c, P<0.05 significant difference vs. B:ICR group.
Figure 4
Figure 4. The oral glucose tolerance test (OGTT) in multiple low dose streptozotocin induced diabetic ICR mice. The data shown represent the means±SD (n=7 per group). a, P<0.05 significant difference vs. Korl:ICR group. b, P<0.05 significant difference vs. A:ICR group. c, P<0.05 significant difference vs. B:ICR group.
Figure 5
Figure 5. The histological and insulin immunostaining appearances of pancreas in multiple low dose streptozotocin induced diabetic ICR mice. The pancreas section was stained using hematoxylin and eosin (Vehicle and MLDS). The immunoreactive insulin-producing β-cells in MLDS-induced diabetic mice were remarkably reduced (Vehicle (I) and MLDS (I)). Magnification ×400. Scale bars, 100 µm.
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