Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1986 Sep;103(3):767-76.
doi: 10.1083/jcb.103.3.767.

Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein

N M Kumar, N B Gilula
Comparative Study

Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein

N M Kumar et al. J Cell Biol. 1986 Sep.

Abstract

An extended synthetic oligonucleotide (58-mer) has been used to identify and characterize a human liver gap junction cDNA. The cDNA is 1,574 bases long and contains the entire coding region for a gap junction protein. In vitro translation of the RNA products of this cDNA is consistent with it coding for a 32,022-D protein. Southern blot analysis indicates that the gap junction gene is present as a single copy, and that it can be detected in a variety of organisms using the human liver cDNA as a probe. The human cDNA has been used to screen a rat liver cDNA library, and a rat liver junction cDNA clone has been isolated. The rat liver clone is 1,127 bases in length, and it has strong sequence homology to the human cDNA in the protein-coding region, but less extensive homology in the 3'-untranslated region.

PubMed Disclaimer

References

    1. Plasmid. 1985 Jan;13(1):31-40 - PubMed
    1. J Cell Biol. 1967 Jun;33(3):C7-C12 - PubMed
    1. EMBO J. 1983;2(9):1479-86 - PubMed
    1. J Cell Biol. 1982 Jan;92(1):53-9 - PubMed
    1. Anal Biochem. 1980 Apr;103(2):264-71 - PubMed

Publication types

Associated data