Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017:1639:145-149.
doi: 10.1007/978-1-4939-7163-3_14.

Periodic Acid-Schiff Staining with Diastase

Dongtao A Fu  1 , Martha Campbell-Thompson  2
Affiliations

Periodic Acid-Schiff Staining with Diastase

Dongtao A Fu et al. Methods Mol Biol. 2017.

Abstract

Periodic Acid-Schiff (PAS) with diastase (PAS-D) refers to the use of the PAS stain in combination with diastase, which is an enzyme that digests the glycogen. The purpose of using the PAS-D procedure is to differentiate glycogen from other PAS-positive elements in tissue samples. The PAS-D method is also used for periportal liver staining of AAT polymer inclusions that are seen in alpha-1 antitrypsin deficiency disease. Here, we describe the procedure of PAS-D staining in formalin-fixed, paraffin-embedded human liver tissues.

Keywords: Alpha-1 antitrypsin deficiency; Human liver; Periodic Acid-Schiff; Periodic Acid-Schiff reaction-diastase; Special stain.

PubMed Disclaimer

Publication types

LinkOut - more resources