Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2017 Nov;27(6):2001-2016.
doi: 10.1007/s10895-017-2139-6. Epub 2017 Jul 28.

Comparative Study of Novel Fluorescent Cyanine Nucleotides: Hybridization Analysis of Labeled PCR Products Using a Biochip

V E Shershov  1 , S A Lapa  1 , V E Kuznetsova  2 , M A Spitsyn  1 , T O Guseinov  1 , S A Polyakov  1 , A A Stomahin  1 , A S Zasedatelev  1 , A V Chudinov  1
Affiliations
Comparative Study

Comparative Study of Novel Fluorescent Cyanine Nucleotides: Hybridization Analysis of Labeled PCR Products Using a Biochip

V E Shershov et al. J Fluoresc. 2017 Nov.

Abstract

This study investigated the synthesis and substrate properties of Cy5-labeled dUTP derivatives with different substituents, linkers between the dye unit and pyrimidine heterocycle and fluorophore charges. Fluorescently labeled nucleoside triphosphates were studied as substrates using multiplex PCR with Taq and Vent (exo-) DNA polymerases, the typical representatives of the A and B polymerase families. The efficiency of nucleotide incorporation during PCR was assessed with a multi-parameter hybridization analysis using a diagnostic DNA microarray. The hybridization analysis indirectly estimates the incorporation efficiency of dye-labeled nucleotides in multiplex PCR. Our results demonstrated higher efficiencies of substrates with electrically neutral dyes than electropositive and electronegative Cy5 residues.

Keywords: Biochip; Cyanine dyes; Fluorescently labeled nucleotides; Fluorophore charge; PCR.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Nucleic Acids Res. 2006;34(19):5383-94 - PubMed
    1. Cytometry. 1989 Jan;10(1):3-10 - PubMed
    1. Bioorg Med Chem. 2015 Nov 15;23(22):7131-7 - PubMed
    1. Nucleic Acids Res. 2003 May 15;31(10 ):2630-5 - PubMed
    1. Bioconjug Chem. 1999 May-Jun;10(3):529-37 - PubMed

Publication types

MeSH terms

LinkOut - more resources