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Review
. 2017 Aug;28(7-8):377-382.
doi: 10.1007/s00335-017-9711-x. Epub 2017 Jul 29.

Phenotyping first-generation genome editing mutants: a new standard?

Lydia Teboul  1 , Stephen A Murray  2 , Patrick M Nolan  3
Affiliations
Review

Phenotyping first-generation genome editing mutants: a new standard?

Lydia Teboul et al. Mamm Genome. 2017 Aug.

Abstract

The unprecedented efficiency of the CRISPR/Cas9 system in genome engineering has opened the prospect of employing mutant founders for phenotyping cohorts, thus accelerating research projects by circumventing the requirement to generate cohorts using conventional two- or three-generation crosses. However, these first-generation mutants are often genetic mosaics, with a complex and difficult to define genetic make-up. Here, we discuss the potential benefits, challenges and scientific validity of such models.

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