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. 2017 Apr-Jun;12(2):177-187.

Molecular and Morphological Characterizations of Echinococcus granulosus from Human and Animal Isolates in Kashan, Markazi Province, Iran

Mohsen Arbabi  1 , Majid Pirestani  2 , Mahdi Delavari  1 , Hossein Hooshyar  1 , Amir Abdoli  2 , Shahab Sarvi  3
Affiliations

Molecular and Morphological Characterizations of Echinococcus granulosus from Human and Animal Isolates in Kashan, Markazi Province, Iran

Mohsen Arbabi et al. Iran J Parasitol. 2017 Apr-Jun.

Abstract

Background: One of the most important zoonotic helminths in the world is known as Echinococcus granulosus. Different strains of the E. granulosus have been described based on morphological and molecular characterizations, however, there is limited information regarding the characteristics of the phenotypes and genotypes of E. granulosus in Iran.

Methods: The present study was prepared to evaluate the phenotypic and genotypic diversity of E. granulosus isolates collected from human, goat, sheep, and cattle based on 19 standard morphometric parameters and mitochondrial and nuclear genes (CO1, ND1, and ITS1) in Kashan, Markazi Province, Iran during 2013-2014.

Results: The biometric analysis for the 19 characters revealed that the 19 morphometric values of cattle isolates were exceptionally higher than human, goat, and sheep isolates (P<0.05). Molecular analysis confirms the morphological findings. Phylogenic analysis of the CO1, NAD1 and ITS1 genes for all isolates, independent of the host, revealed that the common sheep strain (G1) is traveling among livestock in Kashan and the strains are highly adapted to goats, cattle, sheep, and humans.

Conclusion: Both morphological and molecular results of this study indicated that the only genotype G1 of E. granulosus travels between humans and other intermediate hosts of this parasite in the area study.

Keywords: Echinococcus granulosus; Iran; Molecular phylogenetics; Morphological characterization; PCR-RFLP.

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Figures

Fig. 1:
Fig. 1:
Electrophoresis analysis of ITS(1000 bp), CO1(444 bp) and ND1(830 bp). PCR amplification provided from sheep, goats, cattle and human E. granolusus samples (lane 1,2,: Sheep. 3: goats. 4: cattle. 5,6: Human) compared with the molecular weight marker(lane M, 100bp) and negative control (Lane 7)
Fig. 2:
Fig. 2:
PCR-RFLP analyses of mitochondrial ND1 gene uses the restriction endonuclease Rsa1.1, 2: sheep. 3,4: goats. 5: cattle. 6: human. 7: Negative control (without DNA template). M: DNA ladder 100 bp
Fig. 3:
Fig. 3:
PCR-RFLP analyses of genomic ITS gene uses the restriction endonuclease HaeIII.1,2: sheep 3: goat. 4: cattle. 5: human. M : DNA ladder 100bp
Fig. 4:
Fig. 4:
PCR-RFLP analyses of mitochondrial COX1 gene uses the restriction endonuclease HpaII.1,2: Sheep. 3,4: goats. 5: cattle. 6: human. M:DNA ladder 100 bp
Fig. 5:
Fig. 5:
Phylogenetic relationships among E. granolosus inferred from nucleotide sequences of partial ND1 (A), CO1(B) and ITS(C) isolates from human, sheep, goat and cattle in Kashan, center Iran and other previous registered sequence of different areas. The evolutionary history was inferred using the neighbor-joining method. Number indicates bootstrap values (%) from 2000 replicates. The branches supported by 2000 bootstrap replicates
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