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. 2017 Aug 1:89:5.12.1-5.12.18.
doi: 10.1002/cpps.34.

Preparation of Cell Cultures and Vaccinia Virus Stocks

Catherine A Cotter  1 , Patricia L Earl  1 , Linda S Wyatt  1 , Bernard Moss  1
Affiliations

Preparation of Cell Cultures and Vaccinia Virus Stocks

Catherine A Cotter et al. Curr Protoc Protein Sci. .

Abstract

The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation, titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus purification methods are also included. © 2017 by John Wiley & Sons, Inc.

Keywords: cell culture; modified vaccinia virus Ankara; vaccinia virus.

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