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. 2017 Oct 3;8(7):1457-1464.
doi: 10.1080/21505594.2017.1356541. Epub 2017 Aug 1.

Edwardsiella tarda-induced miRNAs in a teleost host: Global profile and role in bacterial infection as revealed by integrative miRNA-mRNA analysis

Yong-Hua Hu  1   2 , Bao-Cun Zhang  1   2 , Hai-Zhen Zhou  1   2   3 , Xiao-Lu Guan  1   2   3 , Li Sun  1   2
Affiliations

Edwardsiella tarda-induced miRNAs in a teleost host: Global profile and role in bacterial infection as revealed by integrative miRNA-mRNA analysis

Yong-Hua Hu et al. Virulence. .
No abstract available

Keywords: Cynoglossus semilaevis; Edwardsiella tarda; immune defense; infection; miRNA.

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Figures

Figure 1.
Figure 1.
miRNA expression during Edwardsiella tarda infection. (A) Venn diagram showing miRNAs expression at different hours post infection (hpi). The numbers inside the diagram stand for the numbers of miRNAs. (B) Differential expression of miRNAs after bacterial infection. (C and D) Scatter plot of the miRNA expression levels at 12 hpi (C) and 24 hpi (D) in comparison with that at 0 hpi. Red and green spots represent miRNAs significantly upregulated and downregulated, respectively.
Figure 2.
Figure 2.
Quantitative real time PCR (qRT-PCR) analysis of the expression of selected miRNAs and their target mRNAs during Edwardsiella tarda infection (A) and validation of miRNA targets (B). (A)Tongue sole were infected with E. tarda for 0 h, 12 h, and 24 h, and miRNA and mRNA expressions in spleen were determined by qRT-PCR. Expression levels relative to 0 hpi were expressed as bars. Bars stacked together indicate no negative correlation between miRNAs and mRNAs. The names of the mRNAs and miRNAs are shown above and below the bars, respectively. (B) 293T cells were co-transfected with different miRNA mimics and their target mRNA-3′-UTR reporter plasmids. Relative luciferase activity was measured at 24 h after transfection. The experiments were performed 3 times, and values are shown as means ± SEM.
Figure 3.
Figure 3.
Effects of miRNAs and miRNA target genes on Edwardsiella tarda infection. (A) Tongue sole were infected with E. tarda in the presence of the agomir of each of the downregulated miRNAs, or agomir plus the plasmid that overexpresses the target gene of the miRNA. Bacterial numbers in spleen were determined at 48 h post-infection (hpi). (B) Tongue sole were infected with E. tarda in the presence of the antagomir of each of the upregulated miRNAs, or antagomir plus the siRNA against the target genes of the miRNAs. Bacterial numbers in spleen were determined as above. The experiments were performed 3 times, and values are shown as means ± SEM. *P < 0.05, **P < 0.01.
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