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. 2017 Dec;44(12):1613-1625.
doi: 10.1007/s10295-017-1973-5. Epub 2017 Aug 2.

Evolution of copper arsenate resistance for enhanced enargite bioleaching using the extreme thermoacidophile Metallosphaera sedula

Chenbing Ai  1   2 , Samuel McCarthy  2 , Yuting Liang  1   2 , Deepak Rudrappa  2 , Guanzhou Qiu  1 , Paul Blum  3
Affiliations

Evolution of copper arsenate resistance for enhanced enargite bioleaching using the extreme thermoacidophile Metallosphaera sedula

Chenbing Ai et al. J Ind Microbiol Biotechnol. 2017 Dec.

Abstract

Adaptive laboratory evolution (ALE) was employed to isolate arsenate and copper cross-resistant strains, from the copper-resistant M. sedula CuR1. The evolved strains, M. sedula ARS50-1 and M. sedula ARS50-2, contained 12 and 13 additional mutations, respectively, relative to M. sedula CuR1. Bioleaching capacity of a defined consortium (consisting of a naturally occurring strain and a genetically engineered copper sensitive strain) was increased by introduction of M. sedula ARS50-2, with 5.31 and 26.29% more copper recovered from enargite at a pulp density (PD) of 1 and 3% (w/v), respectively. M. sedula ARS50-2 arose as the predominant species and modulated the proportions of the other two strains after it had been introduced. Collectively, the higher Cu2+ resistance trait of M. sedula ARS50-2 resulted in a modulated microbial community structure, and consolidating enargite bioleaching especially at elevated PD.

Keywords: Arsenate resistance; Enargite bioleaching; Extreme thermoacidophile; Metallosphaera sedula; Mutation.

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