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Case Reports
. 2018 Jan 1;9(1):110-120.
doi: 10.1080/21505594.2017.1356537. Epub 2017 Aug 16.

Co-occurrence of 3 different resistance plasmids in a multi-drug resistant Cronobacter sakazakii isolate causing neonatal infections

Lining Shi  1 , Quanhui Liang  2 , Zhe Zhan  3 , Jiao Feng  3 , Yachao Zhao  3 , Yong Chen  1 , Mei Huang  1 , Yigang Tong  3 , Weili Wu  4 , Weijun Chen  4 , Xiaojun Li  1 , Zhe Yin  3 , Jinglin Wang  2 , Dongsheng Zhou  3
Affiliations
Case Reports

Co-occurrence of 3 different resistance plasmids in a multi-drug resistant Cronobacter sakazakii isolate causing neonatal infections

Lining Shi et al. Virulence. .

Abstract

Cronobacter sakazakii 505108 was isolated from a sputum specimen of a neonate with severe pneumonia. C. sakazakii 505108 co-harbors 3 resistance plasmids of the IncHI2, IncX3, and IncFIB incomparability groups, respectively. These 3 plasmids have acquired several accessory modules, which carry an extremely large number of resistance genes, especially including those involved in resistance to carbapenems, aminoglycoside, tetracyclines, and phenicols and sulphonamide/trimethoprim. These plasmid-borne antibiotic resistance genes were associated with insertion sequences, integrons, and transposons, indicating that the assembly and mobilization of the corresponding accessory modules with complex chimera structures are facilitated by transposition and/or homologous recombination. This is the first report of fully sequence plasmids in clinical Cronobacter, which provides a deeper insight into plasmid-mediated multi-drug resistance in Cronobacter from hospital settings.

Keywords: Cronobacter sakazakii; carbapenem resistance; multi-drug resistance; plasmids.

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Figures

Figure 1.
Figure 1.
The MDR-1 region from p505108-MDR and comparison with related regions. Genes are denoted by arrows. Genes, mobile elements and other features are colored based on function classification. Shading denotes regions of homology (> 95% nucleotide identity). Numbers in brackets indicate the nucleotide positions within the corresponding plasmids.
Figure 2.
Figure 2.
The MDR-2 region from p505108-MDR and comparison with related regions. Genes are denoted by arrows. Genes, mobile elements and other features are colored based on function classification. Shading denotes regions of homology (> 95% nucleotide identity). Numbers in brackets indicate the nucleotide positions within the corresponding plasmids.
Figure 3.
Figure 3.
Tn6362 from p505108-MDR and comparison with related regions. Genes are denoted by arrows. Genes, mobile elements and other features are colored based on function classification. Shading denotes regions of homology (> 95% nucleotide identity). Numbers in brackets indicate the nucleotide positions within the corresponding plasmids.
Figure 4.
Figure 4.
The aphA1a regions from p505108-MDR and p505108-T6SS and comparison with related regions. Genes are denoted by arrows. Genes, mobile elements and other features are colored based on function classification. Shading denotes regions of homology (> 95% nucleotide identity). Numbers in brackets indicate the nucleotide positions within the corresponding plasmids. The arrowheads indicated the location of PCR primers and the expected amplicons. See Figure S6 for the PCR results.
See this image and copyright information in PMC

References

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