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. 1978 Jul 7;525(1):87-92.
doi: 10.1016/0005-2744(78)90202-4.

Characterization of sterol-ester hydrolase in Saccharomyces cerevisiae

S Taketani, T Osumi, H Katsuki

Characterization of sterol-ester hydrolase in Saccharomyces cerevisiae

S Taketani et al. Biochim Biophys Acta. .

Abstract

A homgenate of Saccharomyces cerevisiae grown under semi-anaerobic as well as aerobic conditions was found to catalyze the hydrolysis of fatty acid esters of sterols in the presence of Triton X-100. The enzyme levels in cells grown under various conditions were similar and the enzyme had a broad substrate specificity for sterol esters. The enzyme was localized in the mitochondrial fraction for the aerobically grown cells and in the mitochondrial and cytosolic fractions for the semi-anaerobically grown cells.

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