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. 2017 Apr 24;10(4):443.
doi: 10.3390/ma10040443.

Linear Graphene Nanocomposite Synthesis and an Analytical Application for the Amino Acid Detection of Camellia nitidissima Chi Seeds

Jinsheng Cheng  1   2 , Ruimin Zhong  3 , Jiajian Lin  4 , Jianhua Zhu  5 , Weihong Wan  6 , Xinyan Chen  7
Affiliations

Linear Graphene Nanocomposite Synthesis and an Analytical Application for the Amino Acid Detection of Camellia nitidissima Chi Seeds

Jinsheng Cheng et al. Materials (Basel). .

Abstract

Husk derived amino modified linear graphene nanocomposites (aLGN) with a diameter range of 80-300 nm and a length range of 100-300 μm were prepared by a modified Hummers method, ammonia treatment, NaBH₄ reduction and phenylalanine induced assembly processes, etc. The resulting composites were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), scanning electron microscopy (SEM), biological microscope (BM), and X-ray diffraction spectroscopy (XRD), etc. Investigations found that the aLGN can serve as the novel coating of stir bar sorptive extraction (SBSE) technology. By combing this technology with gas chromatography-mass spectrometry (GC-MS), the combined SBSE/GC-MS technology with an aLGN coating can detect seventeen kinds of amino acids of Camellia nitidissima Chi seeds, including Ala, Gly, Thr, Ser, Val, Leu, Ile, Cys, Pro, Met, Asp, Phe, Glu, Lys, Tyr, His, and Arg. Compared to a conventional polydimethylsiloxane (PDMS) coating, an aLGN coating for SBSE exhibited a better thermal desorption performance, better analytes fragmentation depressing efficiencies, higher peak intensities, and superior amino acid discrimination, leading to a practicable and highly distinguishable method for the variable amino acid detection of Camellia nitidissima Chi seeds.

Keywords: Camellia nitidissima Chi; amino acid; linear graphene nanocomposites; stir bar sorptive extraction.

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Conflict of interest statement

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

Figures

Figure 1
Figure 1
Photos of Camellia nitidissima Chi: a;b) fruit; c) fruit flesh; and d) seeds.
Figure 2
Figure 2
TEM images of (a) one piece of aLGN nanocomposites; (b) two pieces of aLGN nanocomposites.
Figure 3
Figure 3
AFM image and depth profile of aLGN on a mica substrate, size 2.138 × 2.138 μm.
Figure 4
Figure 4
SEM images of aLGN nanocomposites.
Figure 5
Figure 5
Total ion chromatogram of aLGN coated SBSE/GC-MS analysis for Camellia Nitidissima Chi seeds.
Figure 6
Figure 6
Possible mechanism for the aLGN formation.
See this image and copyright information in PMC

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