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. 2017 Apr 29;10(5):481.
doi: 10.3390/ma10050481.

Antibacterial Activity of Commercial Dentine Bonding Systems against E. faecalis-Flow Cytometry Study

Monika Lukomska-Szymanska  1 , Magdalena Konieczka  2 , Beata Zarzycka  3 , Barbara Lapinska  4 , Janina Grzegorczyk  5 , Jerzy Sokolowski  6
Affiliations

Antibacterial Activity of Commercial Dentine Bonding Systems against E. faecalis-Flow Cytometry Study

Monika Lukomska-Szymanska et al. Materials (Basel). .

Abstract

Literature presents inconsistent results on the antibacterial activity of dentine bonding systems (DBS). Antibacterial activity of adhesive systems depends on several factors, including composition and acidity. Flow cytometry is a novel detection method to measure multiple characteristics of a single cell: total cell number, structural (size, shape), and functional parameters (viability, cell cycle). The LIVE/DEAD® BacLightTM bacterial viability assay was used to evaluate an antibacterial activity of DBS by assessing physical membrane disruption of bacteria mediated by DBS. Ten commercial DBSs: four total-etching (TE), four self-etching (SE) and two selective enamel etching (SEE) were tested. Both total-etching DBS ExciTE F and OptiBond Solo Plus showed comparatively low antibacterial activity against E. faecalis. The lowest activity of all tested TE systems showed Te-Econom Bond. Among SE DBS, G-ænial Bond (92.24% dead cells) followed by Clearfil S3 Bond Plus (88.02%) and Panavia F 2.0 ED Primer II (86.67%) showed the highest antibacterial activity against E. faecalis, which was comparable to isopropranol (positive control). In the present study, self-etching DBS exhibited higher antimicrobial activity than tested total-etching adhesives against E. faecalis.

Keywords: E. faecalis; antibacterial activity; dental bonding systems; flow cytometry.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Flow cytometry analysis of E. faecalis cell-suspension after 60 min incubation with NaCl (a single experiment example). (a) Cells labelled green (SYTO9+); (b) Cells labelled red (PI).
Figure 2
Figure 2
Flow cytometry analysis of E. faecalis cell-suspension after 60 min incubation with G-ænial Bond (a single experiment example). (a) Cells labelled green (SYTO9+); (b) Cells labelled red (PI).
Figure 3
Figure 3
Image gallery of both: (a) live, labelled green; (b) dead, labelled red and less green cells of E. faecalis.
Figure 4
Figure 4
Assessment of dead bacterial cells [%] after incubation with all tested DBS.
Figure 5
Figure 5
Assessment of dead bacterial cells [%] after incubation–group comparison of types (TE, SE, SEE) of tested DBS.
See this image and copyright information in PMC

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