Density of tumor-infiltrating granzyme B-positive cells predicts favorable prognosis in dogs with transitional cell carcinoma

Abstract

Although tumor-infiltrating lymphocytes (TILs) play a key role in anti-tumor immunity, their involvement in canine transitional cell carcinoma (TCC) is not well-documented. The objective of this study was to investigate the association between TIL number and prognosis in dogs with urinary bladder TCC. Immunohistochemical analysis of CD3 and granzyme B was performed using canine TCC (n=32) and normal bladder (n=10) tissues. The numbers of CD3⁺ and granzyme B⁺ cells located in peritumoral stroma of canine TCC were significantly higher than those in normal controls. In TCC cases, the number of CD3⁺ TILs was not significantly related to prognosis, whereas the abundant granzyme B⁺ TILs were associated with favorable outcome. Since granzyme B⁺ TILs were not associated with the tumor stage, the presence of granzyme B⁺ TILs may be an independent prognostic factor. These results suggest that granzyme B⁺ TILs play a role in anti-tumor immunity and inhibit tumor progression in canine TCC.

Keywords: Anti-tumor immunity; Canine; Tumor-infiltrating lymphocytes; Urinary bladder; Urothelial carcinoma.