Neural and paracrine regulation of gastrin release using rat antral mucosa in tissue culture--the effect of carbachol, bombesin, and anti-somatostatin antibody on gastrin release

Abstract

Gastrin release was significantly stimulated by the cholinergic agent carbachol at doses of 10(-4) M, 10(-5) M, and 10(-6) M. Peak stimulation was observed at 10(-5) M. Gastrin release was also significantly stimulated by bombesin at a dose of 10(-8) M, and 10(-6) M atropine which abolished the effect of carbachol in stimulating gastrin release had no effect on the bombesin-stimulated gastrin release. In addition, anti-somatostatin antiserum significantly stimulated gastrin release. These findings suggest that gastrin release is regulated by cholinergic and noncholinergic neurons the latter being thought to be a bombesin-containing neuron, and that antral somatostatin exerts a continuous restraint on gastrin release by the paracrine mechanism.