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. 2017 Aug;14(2):2305-2309.
doi: 10.3892/ol.2017.6423. Epub 2017 Jun 20.

Expression and prognostic significance of miR-375 and miR-221 in liver cancer

Dafei Xie  1 , Peiwen Yuan  2 , Dong Wang  1 , Hua Jin  1 , Hui Chen  1
Affiliations

Expression and prognostic significance of miR-375 and miR-221 in liver cancer

Dafei Xie et al. Oncol Lett. 2017 Aug.

Abstract

The purpose of this study was to investigate the expression of miR-375 and miR-221 in liver cancer, and examine the correlations with pathological parameters and prognosis. We collected tumors and tumor-adjacent normal tissue from 70 patients with liver cancer admitted to the Department of General Surgery of Zhejiang Hospital. The expression of miR-375 by RT-qPCR was significantly lower in liver cancer tissues than that in the tumor-adjacent normal tissues, and the low expression was correlated with the lymphatic metastasis and TNM stage. By contrast, the expression of miR-221 was significantly higher in liver cancer than that in the tumor-adjacent tissues, and the high expression was correlated with the lymphatic metastasis and TNM stage. The overall 5-year survival rate of patients was 12.9% (9/70). Single-factor survival analysis revealed that miR-375 and miR-221 were the factors affecting the overall survival rate of liver cancer (P<0.05) and multivariate survival analysis by Cox proportional hazards model showed that miR-375 and miR-221 were the independent factors affecting the survival time of patients with liver cancer. Low expression of miR-375 and high expression of miR-221 are closely correlated with the occurrence and development of liver cancer, especially lymphatic metastasis and TNM stage. Thus, miR-375 and miR-221 can serve as reference biomarkers for guiding the treatment of liver cancer and for estimating prognosis.

Keywords: liver cancer; miR-221; miR-375; prognosis.

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Figures

Figure 1.
Figure 1.
Expression of miR-375 and miR-221 in specimens by RT-qPCR. Compared with the tumor-adjacent normal tissues, the expression of miR-375 was significantly decreased in the liver cancer tissues, but the expression of miR-221 was obviously elevated. Expression of (A) miR-375 and (B) miR-221 in tissue sample. Compared with the tumor-adjacent normal tissues, **P<0.01.
Figure 2.
Figure 2.
Expression of miR-375 and miR-221 and Kaplan-Meier survival curves of patients with liver cancer. (A) Kaplan-Meier survival curves of liver cancer patients with a high and low expression of miR-375. (B) Kaplan-Meier survival curves of liver cancer patients with a high and low expression of miR-221.
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