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. 2017 Aug 7;6(8):78.
doi: 10.3390/jcm6080078.

Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis

Sharlaa Badal-Faesen  1 , Cynthia Firnhaber  2 , Michelle A Kendall  3 , Xingye Wu  4 , Beatriz Grinsztejn  5 , Rodrigo Otavio da Silva Escada  6 , Michel Fernandez  7 , Evelyn Hogg  8 , Ian Sanne  9 , Pamela Johnson  10 , David Alland  11 , Gerald H Mazurek  12 , Debra A Benator  13 , Anne F Luetkemeyer  14 ; AIDS Clinical Trials Group A5295  2 ; Tuberculosis Trials Consortium Study 34 Teams  9
Affiliations

Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis

Sharlaa Badal-Faesen et al. J Clin Med. .

Abstract

As a strategy to improve the sensitivity of nucleic acid-based testing in acid-fast bacilli (AFB) negative samples, larger volumes of sputum (5-10 mL) were tested with Xpert® MTB/RIF from 176 individuals with smear-negative sputum undergoing tuberculosis evaluation. Despite larger volumes, this strategy had a suboptimal sensitivity of 50% (4/8).

Keywords: Xpert MTB/RIF; larger volume sputum; smear-negative suspects.

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Conflict of interest statement

D.A. has received contracts for research from Cepheid, and is one of a group of coinvestigators who invented molecular beacon technology and who receive income from licensees, including a license to Cepheid for M. tuberculosis detection. However, the income attributable to the Xpert MTB/RIF assay which he may receive has been irrevocably capped at $5000 per year as a management of this conflict of interest. P.J. is an employee of Cepheid. D.A., D.A.B., C.F., and A.F.L. have received research grant support from Cepheid. All other authors report no potential conflicts.

References

    1. World Health Organization Global Tuberculosis Report 2015. [(accessed on 16 March 2016)]; Available online: http://www.who.int/tb/publications/global_report/en/
    1. Steingart K.R., Schiller I., Horne D.J., Pai M., Boehme C.C., Dendukuri N. Xpert(R) MTB/RIF assay for pulmonary tuberculosis and rifampicin resistance in adults. Cochrane Database Syst. Rev. 2014;1:CD009593. - PMC - PubMed
    1. Luetkemeyer A.F., Firnhaber C., Kendall M.A., Wu X., Mazurek G.H., Benator D.A, Arduino R., Fernandez M., Guy E., Johnson P., et al. Evaluation of Xpert MTB/RIF Versus AFB Smear and Culture to Identify Pulmonary Tuberculosis in Patients with Suspected Tuberculosis from Low and Higher Prevalence Settings. Clin. Infect. Dis. 2016;62:1081–1088. doi: 10.1093/cid/ciw035. - DOI - PMC - PubMed
    1. Warren J.R., Bhattacharya M., De Almeida K.N., Trakas K., Peterson L.R. A minimum 5.0 mL of sputum improves the sensitivity of acid-fast smear for Mycobacterium tuberculosis. Am. J. Respir. Crit. Care Med. 2000;161:1559–1562. doi: 10.1164/ajrccm.161.5.9908063. - DOI - PubMed
    1. Alisjahbana B., van Crevel R., Danusantoso H., Gartinah T., Soemantri E.S., Nelwan R.H., van der Meer J.W. Better patient instruction for sputum sampling can improve microscopic tuberculosis diagnosis. Int. J. Tuberc. Lung Dis. 2005;9:814–817. - PubMed

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