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Comment
. 2017 Aug 8;8(4):e01000-17.
doi: 10.1128/mBio.01000-17.

Observed High Coinfection Rates Seem To Be a Result of Overlapping Plaques

Nir Drayman  1
Affiliations
Comment

Observed High Coinfection Rates Seem To Be a Result of Overlapping Plaques

Nir Drayman. mBio. .
No abstract available

Keywords: methodology; plaque assay; poliovirus.

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Figures

FIG 1
FIG 1
Simulations of plaque assays show high prevalence of overlapping plaques. (A) Example of a virtual plaque assay. Each circle is a single plaque. This example shows 20 plaques, each with a 2-mm diameter, four of which overlap. (B) Percentages of overlapping plaques in 1,000 simulations for each combination of plaque size and number of plaques per plate. (C) P values calculated for observing >5% coinfection rates when “picking” 120 plaques under each condition.
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References

    1. Aguilera ER, Erickson AK, Jesudhasan PR, Robinson CM, Pfeiffer JK. 2017. Plaques formed by mutagenized viral populations have elevated coinfection frequencies. mBio 8:e02020-16. doi: 10.1128/mBio.02020-16. - DOI - PMC - PubMed
    1. Dulbecco R, Vogt M. 1954. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med 99:167–182. doi: 10.1084/jem.99.2.167. - DOI - PMC - PubMed

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