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Comparative Study
. 2017 Jul;72(7):400-404.
doi: 10.6061/clinics/2017(07)02.

Validation of QF-PCR for prenatal diagnoses in a Brazilian population

Renata Wendel de Moraes  1 , Mario Henrique Burlacchini de Carvalho  1 , Antonio Gomes de Amorim-Filho  1 , Rossana Pulcineli Vieira Francisco  1 , Renata Moscolini Romão  1 , José Eduardo Levi  2 , Marcelo Zugaib  1
Affiliations
Comparative Study

Validation of QF-PCR for prenatal diagnoses in a Brazilian population

Renata Wendel de Moraes et al. Clinics (Sao Paulo). 2017 Jul.

Abstract

Objectives:: Quantitative fluorescence polymerase chain reaction (QF-PCR) is a rapid and reliable method for screening aneuploidies, but in Brazil, it is not used in public services. We investigated the accuracy of QF-PCR for the prenatal recognition of common aneuploidies and compared these results with cytogenetic results in our laboratory.

Method:: A ChromoQuant QF-PCR kit containing 24 primer pairs targeting loci on chromosomes 21, 13, 18, X and Y was employed to identify aneuploidies of the referred chromosomes.

Results:: A total of 162 amniotic fluid samples analyzed using multiplex QF-PCR were compared with karyotyping analysis. The QF-PCR results were consistent with the results of cytogenetic analysis in 95.4% of all samples.

Conclusion:: QF-PCR was demonstrated to be efficient and reliable for prenatal aneuploidy screening. This study suggests that QF-PCR can be used as a rapid diagnostic method. However, rearrangements and some mosaic samples cannot be detected with this test; thus, those exceptions must undergo cytogenetic analysis.

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Conflict of interest statement

No potential conflict of interest was reported.

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