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. 2017 Aug;7(4):274.
doi: 10.1007/s13205-017-0907-2. Epub 2017 Aug 2.

High frequency direct shoot organogenesis of leaf explants and a comparative evaluation of phytochemicals, antioxidant potential of wild vs. in vitro plant extracts of Lysimachia laxa

Sanjoy Gupta  1   2 , Tapan Seal  3 , A A Mao  1 , Soneswar Sarma  4
Affiliations

High frequency direct shoot organogenesis of leaf explants and a comparative evaluation of phytochemicals, antioxidant potential of wild vs. in vitro plant extracts of Lysimachia laxa

Sanjoy Gupta et al. 3 Biotech. 2017 Aug.

Abstract

The present studies were attempted to develop direct shoot organogenesis from in vitro grown leaf explants of Lysimachia laxa and comparative evaluation of phytochemical and antioxidant potential of in vitro raised and wild plants extracts. The fresh leaves of this species are used for deworming gastrointestinal worm infection in traditional medicine. Overexploitation of this species and poor regeneration has led to rapid decline in wild population, therefore, present investigation was attempted to develop an efficient rapid mass propagation protocol for this species. Our result showed significantly (P < 0.05) high adventitious shoot proliferation of 17.21 ± 0.24 number per leaf explants cultured in Murashige and Skoog medium fortified with 1.25 mg L-1 thiadizuron and 1.0 mg L-1 α-naphthalene acetic acid. Further enhancement was achieved through elongation medium fortified with 1.0 mg L-1 6-benzylaminopurine by average shoot number of 31.1 ± 0.80 and length of 5.96 ± 0.13 cm. Murashige and Skoog medium fortified with 0.50 mg L-1 Indole-3-acetic acid showed high rooting induction (100%) with average root number of 11.70 and length 7.35 cm. All rooted plants were successfully acclimatized in greenhouse and transferred to field condition with a survival rate of 97%. The contents of phenolic and flavonoid were higher in in vitro raised plant in compared to wild plant extracts. Antioxidants assay showed high radical scavenging activity of IC50 1.61 ± 0.07 mg dry material and reducing power of 49.79 ± 0.11 mg/g ascorbic acid equivalent by aqueous methanol extracts of in vitro raised 3-months-old plants in compare to the wild plants. The present protocol is a viable option for pharmaceutical or nutraceutical industries for sustainable utilization of L. laxa with enhanced of phytochemical and antioxidant potency which is not reported elsewhere.

Keywords: Antioxidant; Direct organogenesis; In vitro and wild plant extract; Lysimachia laxa; Phytochemicals.

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Conflict of interest statement

All authors declare that they have no any conflict of interest in this publication. This publication is approved by the author and all coauthors. The work was carried out at Plant Tissue culture Lab, Botanical Survey of India, ERC, Shillong.

Figures

Fig. 1
Fig. 1
Direct organogenesis from leaf explants of L. laxa. a Initiation start on the surface of leaf lamina after 2nd week cultured. b Shoots proliferation in MS medium fortified with TDZ + NAA (1.25 + 1 mg L−1) after 4th week cultured. c Shoot buds elongation and proliferation in MS medium fortified with 1.50 mg L−1 BA after 7th week cultured. d Arrow mark fasciation shoot at the concentration of 2 mg L−1 TDZ after 8th week cultured. e Rooting in MS medium fortified with 0.50 mg L−1 IAA after 6 weeks. f Rooting in control after 6 weeks cultured. g 3-months-old under greenhouse condition. h Acclimatized plants after 4 months in natural condition. In all figures showing scale bar in 1 cm length excepting figs. g and h scale bar 5 cm in length
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References

    1. Abbasi BH, Khan MA, Mahmood T, Ahmad M, Chaudhary MF, Khan MA. Shoot regeneration and free-radical scavenging activity in Silybum marianum L. Plant Cell Tissue Organ Cult. 2010;101:371–376. doi: 10.1007/s11240-010-9692-x. - DOI
    1. Aprotosoaie AC, Mihai CT, Vochita G, Rotinberg P, Trifan A, Luca SV, Petreus T, Gille E, Miron A. Antigenotoxic and antioxidant activities of a polyphenolic extract from European Dracocephalum moldavica L. Ind Crops Prod. 2016;79:248–257. doi: 10.1016/j.indcrop.2015.11.004. - DOI
    1. Baskaran P, Moyo M, Van Staden J. In vitro plant regeneration, phenolic compound production and pharmacological activities of Coleonema pulchellum. S Afr J Bot. 2014;90:74–79. doi: 10.1016/j.sajb.2013.10.005. - DOI
    1. Berker KI, Guçlu K, Tor I, Apak R. Comparative evaluation of Fe (III) reducing power-based antioxidant capacity assays in the presence of phenanthroline, batho-phenanthroline, tripyridyltriazine (FRAP), and ferricyanide reagents. Talanta. 2007;72:1157–1165. doi: 10.1016/j.talanta.2007.01.019. - DOI - PubMed
    1. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181:1199–1200. doi: 10.1038/1811199a0. - DOI

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