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. 2017 Aug 10;10(8):927.
doi: 10.3390/ma10080927.

Dehydrothermally Cross-Linked Collagen Membrane with a Bone Graft Improves Bone Regeneration in a Rat Calvarial Defect Model

Yin-Zhe An  1 , Young-Ku Heo  2 , Jung-Seok Lee  3 , Ui-Won Jung  4 , Seong-Ho Choi  5
Affiliations

Dehydrothermally Cross-Linked Collagen Membrane with a Bone Graft Improves Bone Regeneration in a Rat Calvarial Defect Model

Yin-Zhe An et al. Materials (Basel). .

Abstract

In this study, the bone regeneration efficacy of dehydrothermally (DHT) cross-linked collagen membrane with or without a bone graft (BG) material was evaluated in a critical-sized rat model. An 8-mm-diameter defect was created in the calvaria of 40 rats, which were randomized into four groups: (1) control; (2) DHT; (3) BG; and, (4) DHT + BG. Evaluations were made at 2 and 8 weeks after surgery using micro-computed tomographic (micro-CT), histological, and histomorphometric analyses. Micro-CT analysis showed an increase in the new bone volume (NBV) of the BG and DHT + BG groups at 2 weeks after surgery, representing a significant difference (p < 0.05). At 8 weeks after surgery, the NBV increased in all four groups. However, larger NBVs were observed in the BG and DHT + BG groups, and a significant difference was no longer observed between the two groups. Histologic analysis demonstrated that the graft materials sustained the center of the defect in the BG and DHT + BG groups, which was shown in histomorphometric analysis as well. These results suggest that DHT membrane is a safe biomaterial with adequate tissue integration, and has a positive effect on new bone formation. Moreover, the best effects were achieved when DHT was used in conjunction with BG materials.

Keywords: allograft; bone regeneration; collagen; cross-linking; dehydrothermal.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Three-dimensional reconstruction images obtained at 2 and 8 weeks after surgery. (a,e) Control group; (b,f) DHT membrane group; (c,g) BG group; (d,h) DHT + BG group. Gray: mother bone; green: new bone; purple: bone graft material.
Figure 2
Figure 2
Histologic transversal sections obtained at 2 weeks after surgery (hematoxylin and eosin staining). (a,b) Control group; (c,d) DHT membrane group; (e,f) BG group; (g,h) DHT + BG group. The boxed areas in the left panels (40× magnification) are magnified in the corresponding panels on the right (200×). Arrowhead: defect margin. MB: mother bone; NB: new bone; CM: collagen membrane; RM: residual material; black arrow: vascular endothelial cells.
Figure 3
Figure 3
Histologic transversal sections obtained at 8 weeks after surgery (hematoxylin and eosin staining). (a,b) Control group; (c,d) DHT membrane group; (e,f) BG group; (g,h) DHT + BG group. The boxed areas in the left panels (40× magnification) are magnified in the corresponding panels on the right (200×). Arrowhead: defect margin. CT: connective tissue; NB (black arrow): new bone; CM: collagen membrane; RM: residual material.
Figure 4
Figure 4
Expression patterns of proliferating cell nuclear antigen (PCNA) in tissue sections detected by immunohistochemistry: example of a negative (a,b) and positive (c,d) patterns of PCNA expression in tissue sections. The boxed areas in the left panels (200× magnification) are magnified in the corresponding panels on the right (1000× magnification).
See this image and copyright information in PMC

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