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Review
. 2017 Nov;15(11):1361-1370.
doi: 10.1111/pbi.12805. Epub 2017 Sep 11.

Novel technologies in doubled haploid line development

Jiaojiao Ren  1   2 , Penghao Wu  3 , Benjamin Trampe  2 , Xiaolong Tian  1 , Thomas Lübberstedt  2 , Shaojiang Chen  1
Affiliations
Review

Novel technologies in doubled haploid line development

Jiaojiao Ren et al. Plant Biotechnol J. 2017 Nov.

Abstract

haploid inducer line can be transferred (DH) technology can not only shorten the breeding process but also increase genetic gain. Haploid induction and subsequent genome doubling are the two main steps required for DH technology. Haploids have been generated through the culture of immature male and female gametophytes, and through inter- and intraspecific via chromosome elimination. Here, we focus on haploidization via chromosome elimination, especially the recent advances in centromere-mediated haploidization. Once haploids have been induced, genome doubling is needed to produce DH lines. This study has proposed a new strategy to improve haploid genome doubling by combing haploids and minichromosome technology. With the progress in haploid induction and genome doubling methods, DH technology can facilitate reverse breeding, cytoplasmic male sterile (CMS) line production, gene stacking and a variety of other genetic analysis.

Keywords: chromosome elimination; doubled haploid; genome doubling; haploidization; minichromosome.

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Figures

Figure 1
Figure 1
Proposed model of chromosome elimination in H. vulgare × H. bulbosum.
Figure 2
Figure 2
Two possible mechanisms of in vivo haploid induction in maize.
Figure 3
Figure 3
A model for the process of haploid induction via modification of CENH3.
See this image and copyright information in PMC

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