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. 2017 Aug 3;22(31):30589.
doi: 10.2807/1560-7917.ES.2017.22.31.30589.

Novel plasmid-mediated colistin resistance mcr-4 gene in Salmonella and Escherichia coli, Italy 2013, Spain and Belgium, 2015 to 2016

Alessandra Carattoli  1 , Laura Villa  1 , Claudia Feudi  1   2 , Ludovica Curcio  3 , Serenella Orsini  3 , Andrea Luppi  4 , Giovanni Pezzotti  3 , Chiara Francesca Magistrali  3
Affiliations

Novel plasmid-mediated colistin resistance mcr-4 gene in Salmonella and Escherichia coli, Italy 2013, Spain and Belgium, 2015 to 2016

Alessandra Carattoli et al. Euro Surveill. .

Abstract

A novel mcr colistin resistance gene was identified in a strain of Salmonella enterica, monophasic variant of serovar Typhimurium (4,5,12:i:- ), isolated from a pig at slaughter in Italy in 2013, and in Escherichia coli strains collected during routine diagnostic of post-weaning diarrhoea in pigs from Spain and Belgium in 2015 and 2016. Immediate implementation of mcr-screening including this novel gene variant is required for Salmonella and E. coli from humans and food-producing animals in Europe.

Keywords: ColE; Salmonella; colistin; mcr.

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Conflict of interest statement

Conflict of interest: None declared.

Figures

Figure 1
Figure 1
Plasmid content of the Salmonella isolate from swine, Italy 2013 (n = 1) and its transformant 3445T and map of the pMCR plasmid carrying the mcr-4 gene
Figure 2
Figure 2
Phylogenetic analysis of the entire MCR-4 protein sequence
See this image and copyright information in PMC

References

    1. Rhouma M, Beaudry F, Letellier A. Resistance to colistin: what is the fate for this antibiotic in pig production? Int J Antimicrob Agents. 2016;48(2):119-26. 10.1016/j.ijantimicag.2016.04.008 - DOI - PubMed
    1. Pesciaroli M, Cucco L, De Luca S, Massacci FR, Maresca C, Medici L, et al. Association between pigs with high caecal Salmonella loads and carcass contamination. Int J Food Microbiol. 2017;242:82-6. 10.1016/j.ijfoodmicro.2016.11.021 - DOI - PubMed
    1. European Committee on Antimicrobial Susceptibility Testing (EUCAST). Clinical Breakpoints. Växjö: EUCAST. Jun 2017. Available from: http://eucast.org/clinical_breakpoints
    1. Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S, Lund O, et al. Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother. 2012;67(11):2640-4. 10.1093/jac/dks261 - DOI - PMC - PubMed
    1. Carattoli A, Zankari E, García-Fernández A, Voldby Larsen M, Lund O, Villa L, et al. In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing. Antimicrob Agents Chemother. 2014;58(7):3895-903. 10.1128/AAC.02412-14 - DOI - PMC - PubMed