Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Jan;24(1):39-46.
doi: 10.1136/jmg.24.1.39.

High resolution gene mapping of the human alpha globin locus

R D Nicholls, J A Jonasson, J O McGee, S Patil, V V Ionasescu, D J Weatherall, D R Higgs

High resolution gene mapping of the human alpha globin locus

R D Nicholls et al. J Med Genet. 1987 Jan.

Abstract

A combination of polymorphic DNA markers, cytogenetic analysis, and in situ hybridisation has been used for the high resolution assignment of the human alpha globin gene cluster on chromosome 16. Multiallelic DNA probes from within the alpha globin cluster were used to determine the number of copies of this locus in three cell lines containing trisomies of the short arm of chromosome 16 and one with a familial inversion, inv(16). The breakpoints in these rearrangements flank the alpha globin locus and locate a shortest region of overlap to 16p13.1. A meiotic crossover was also localised to this band. In situ hybridisation of biotinylated DNA probes to normal and inverted chromosomes 16 [inv(16)(p13.1;q22)] showed hybridisation sites at opposite ends of the chromosomes, consistent with this regional localisation.

PubMed Disclaimer

References

    1. Nature. 1985 Jul 25-31;316(6026):330-4 - PubMed
    1. EMBO J. 1986 Aug;5(8):1857-63 - PubMed
    1. Am J Hum Genet. 1985 Sep;37(5):965-72 - PubMed
    1. J Clin Pathol. 1985 Oct;38(10):1085-92 - PubMed
    1. Nucleic Acids Res. 1985 Nov 11;13(21):7569-78 - PubMed

Publication types

LinkOut - more resources