A Modular Approach to Building Complex Synthetic Circuits

Abstract

Efficient assembly of genetic circuits is a critical element in synthetic biology for implementing complex gene expression systems. Here we present an assembly framework that integrates recent developments in DNA recombination technologies and unique nucleotide sequences for modular and reliable construction of complex genetic circuits. All relevant protocols are described in detail. Implementing these protocols relies on readily available reagents and basic molecular biology techniques without the need for specialized equipment.

Keywords: Gateway recombination; Gene expression; Genetic circuits; Gibson Assembl y; Hierarchical assembly; Modular assembly; Synthetic biology.