Acute regulated expression of pendrin in human urinary exosomes

Abstract

It is well known that pendrin, an apical Cl^-/HCO₃^-exchanger in type B intercalated cells, is modulated by chronic acid-base disturbances and electrolyte intake. To study this adaptation further at the acute level, we analyzed urinary exosomes from individuals subjected to oral acute acid, alkali, and NaCl loading. Acute oral NH₄Cl loading (n = 8) elicited systemic acidemia with a drop in urinary pH and an increase in urinary NH₄ excretion. Nadir urinary pH was achieved 5 h after NH₄Cl loading. Exosomal pendrin abundance was dramatically decreased at 3 h after acid loading. In contrast, after acute equimolar oral NaHCO₃ loading (n = 8), urinary and venous blood pH rose rapidly with a significant attenuation of urinary NH₄ excretion. Alkali loading caused rapid upregulation of exosomal pendrin abundance at 1 h and normalized within 3 h of treatment. Equimolar NaCl loading (n = 6) did not alter urinary or venous blood pH or urinary NH₄ excretion. However, pendrin abundance in urinary exosomes was significantly reduced at 2 h of NaCl ingestion with lowest levels observed at 4 h after treatment. In patients with inherited distal renal tubular acidosis (dRTA), pendrin abundance in urinary exosomes was greatly reduced and did not change upon oral NH₄Cl loading. In summary, pendrin can be detected and quantified in human urinary exosomes by immunoblotting. Acid, alkali, and NaCl loadings cause acute changes in pendrin abundance in urinary exosomes within a few hours. Our data suggest that exosomal pendrin is a promising urinary biomarker for acute acid-base and volume status changes in humans.

Keywords: Acid-base homeostasis; Distal renal tubular acidosis; Pendrin; SLC26A4; Urinary exosomes.