Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood

doi:10.1007/978-1-4939-7196-1_28

. 2017:1646:369-389.

doi: 10.1007/978-1-4939-7196-1_28.

Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood

Leonardo Pasalic^{1

2}, Gabrielle J Pennings³, David Connor⁴, Heather Campbell⁵, Leonard Kritharides^{3

6}, Vivien M Chen^{3

5

7}

Affiliations

¹ Departments of Clinical and Laboratory Haematology, Institute of Clinical Pathology and Medical Research, WestmeadHospital, Westmead, NSW 2145, Australia. leonardo.pasalic@health.nsw.gov.au.
² Sydney Centres for Thrombosis and Haemostasis, Westmead, NSW, Australia. leonardo.pasalic@health.nsw.gov.au.
³ ANZAC Research Institute, University of Sydney, Sydney, NSW, Australia.
⁴ Blood, Stem Cell and Cancer Research Unit, St Vincent's Centre for Applied Medical Research, Sydney, NSW, Australia.
⁵ Prince of Wales Clinical School, Faculty of Medicine, University Of New South Wales, Sydney, NSW, Australia.
⁶ Department of Cardiology, Concord Repatriation General Hospital, Sydney, NSW, Australia.
⁷ Department of Haematology, Concord Repatriation General Hospital, Sydney, NSW, Australia.

PMID: 28804842
DOI: 10.1007/978-1-4939-7196-1_28

Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood

Leonardo Pasalic et al. Methods Mol Biol. 2017.

. 2017:1646:369-389.

doi: 10.1007/978-1-4939-7196-1_28.

Authors

Leonardo Pasalic^{1

2}, Gabrielle J Pennings³, David Connor⁴, Heather Campbell⁵, Leonard Kritharides^{3

6}, Vivien M Chen^{3

5

7}

Affiliations

¹ Departments of Clinical and Laboratory Haematology, Institute of Clinical Pathology and Medical Research, WestmeadHospital, Westmead, NSW 2145, Australia. leonardo.pasalic@health.nsw.gov.au.
² Sydney Centres for Thrombosis and Haemostasis, Westmead, NSW, Australia. leonardo.pasalic@health.nsw.gov.au.
³ ANZAC Research Institute, University of Sydney, Sydney, NSW, Australia.
⁴ Blood, Stem Cell and Cancer Research Unit, St Vincent's Centre for Applied Medical Research, Sydney, NSW, Australia.
⁵ Prince of Wales Clinical School, Faculty of Medicine, University Of New South Wales, Sydney, NSW, Australia.
⁶ Department of Cardiology, Concord Repatriation General Hospital, Sydney, NSW, Australia.
⁷ Department of Haematology, Concord Repatriation General Hospital, Sydney, NSW, Australia.

PMID: 28804842
DOI: 10.1007/978-1-4939-7196-1_28

Abstract

Flow cytometry is a powerful tool for rapid evaluation of multiple functional properties of large numbers of platelets in whole blood. In the following chapter, we provide a number of flow cytometry-based protocols broadly aimed at (1) assessment of constitutively expressed platelet membrane receptors to diagnose inherited platelet bleeding disorders and (2) investigation of basal and agonist-induced platelet functional responses including generation of platelet-leukocyte aggregates, alpha and dense granule release, calcium flux, and phosphatidylserine exposure.

Keywords: Calcium flux; Dense granule release; Flow cytometry; Glycoprotein receptor deficiency; Platelet function; Platelet-leukocyte aggregates; Procoagulant platelets; Whole blood.

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Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood

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Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood

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