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. 2017:1646:369-389.
doi: 10.1007/978-1-4939-7196-1_28.

Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood

Leonardo Pasalic  1   2 , Gabrielle J Pennings  3 , David Connor  4 , Heather Campbell  5 , Leonard Kritharides  3   6 , Vivien M Chen  3   5   7
Affiliations

Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood

Leonardo Pasalic et al. Methods Mol Biol. 2017.

Abstract

Flow cytometry is a powerful tool for rapid evaluation of multiple functional properties of large numbers of platelets in whole blood. In the following chapter, we provide a number of flow cytometry-based protocols broadly aimed at (1) assessment of constitutively expressed platelet membrane receptors to diagnose inherited platelet bleeding disorders and (2) investigation of basal and agonist-induced platelet functional responses including generation of platelet-leukocyte aggregates, alpha and dense granule release, calcium flux, and phosphatidylserine exposure.

Keywords: Calcium flux; Dense granule release; Flow cytometry; Glycoprotein receptor deficiency; Platelet function; Platelet-leukocyte aggregates; Procoagulant platelets; Whole blood.

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