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. 2017 Aug 14;14(1):152.
doi: 10.1186/s12985-017-0817-2.

Quantitative detection of human Malawi polyomavirus in nasopharyngeal aspirates, sera, and feces in Beijing, China, using real-time TaqMan-based PCR

Fen-Lian Ma  1 , Dan-di Li  1 , Tian-Li Wei  2 , Jin-Song Li  1 , Li-Shu Zheng  3
Affiliations

Quantitative detection of human Malawi polyomavirus in nasopharyngeal aspirates, sera, and feces in Beijing, China, using real-time TaqMan-based PCR

Fen-Lian Ma et al. Virol J. .

Abstract

Background: Human Malawi polyomavirus (MWPyV) was discovered in 2012, but its prevalence and clinical characteristics are largely unknown.

Methods: We used real-time TaqMan-based PCR to detect MWPyV in the feces (n = 174) of children with diarrhea, nasopharyngeal aspirates (n = 887) from children with respiratory infections, and sera (n = 200) from healthy adults, and analyzed its clinical characteristics statistically. All the MWPyV-positive specimens were also screened for other common respiratory viruses.

Results: Sixteen specimens were positive for MWPyV, including 13 (1.47%) respiratory samples and three (1.7%) fecal samples. The samples were all co-infected with other respiratory viruses, most commonly with influenza viruses (69.2%) and human coronaviruses (30.7%). The MWPyV-positive children were diagnosed with bronchopneumonia or viral diarrhea. They ranged in age from 12 days to 9 years, and the most frequent symptoms were cough and fever.

Conclusions: Real-time PCR is an effective tool for the detection of MWPyV in different types of samples. MWPyV infection mainly occurs in young children, and fecal-oral transmission is a possible route of its transmission.

Keywords: Feces; Human Malawi polyomavirus (MWPyV); Nasopharyngeal aspirate; Respiratory virus; TaqMan real-time PCR.

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Conflict of interest statement

Ethics approval and consent to participate

The project was approval by the Ethical Committee of the Beijing Friendship Hospital, China, Capital Institute of Pediatrics, China and Institute of Blood Transfusion, Chinese Academy Medical Sciences.

Consent for publication

Written informed consent for specimen collection, testing and publication was obtained from the patients’ parents or guardians.

Competing interests

The authors declare that they have no competinginterest.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
a Age distribution and the percentage of MWPyV-positive children with ARIs; b Seasonal distribution and the percentage of MWPyV-positive children with ARIs
See this image and copyright information in PMC

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