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. 2017 Jan 19;31(3):240-247.
doi: 10.7555/JBR.31.20160150.

Modeling and validating three dimensional human normal cervix and cervical cancer tissues in vitro

Anna Karolina Zuk  1 , Xuesong Wen  1 , Stephen Dilworth  1 , Dong Li  1 , Lucy Ghali  1
Affiliations

Modeling and validating three dimensional human normal cervix and cervical cancer tissues in vitro

Anna Karolina Zuk et al. J Biomed Res. .

Abstract

The use of three dimensional in vitro systems in cancer research is a promising path for developing effective anticancer therapies. The aim of this study was to engineer a functional 3-Din vitro model of normal and cancerous cervical tissue.Normal epithelial and immortalized cervical epithelial carcinoma cell lines were used to construct 3-D artificial normal cervical and cervical cancerous tissues. De-epidermised dermis (DED) was used as a scaffold for both models. Morphological analyses were conducted by using hematoxylin and eosin staining and characteristics of the models were studied by analyzing the expression of different structural cytokeratins and differential protein marker Mad1 using immunohistochemical technique.Haematoxylin and eosin staining results showed that normal cervical tissue had multi epithelial layers while cancerous cervical tissue showed dysplastic changes. Immunohistochemistry staining results revealed that for normal cervix model cytokeratin 10 was expressed in the upper stratified layer of epithelium while cytokeratin 5 was expressed mainly in the middle and basal layer. Cytokeratin 19 was weakly expressed in a few basal cells. Cervical cancer model showed cytokeratin 19 expression in different epithelial layers and weak or no expression for cytokeratin 5 and cytokeratin 10. Mad1 expression was detected in some suprabasal cells.The 3-Din vitro models showed stratified epithelial layers and expressed the same types and patterns of differentiation marker proteins as seen in correspondingin vivo tissue in either normal cervical or cervical cancerous tissue. Findings imply that they can serve as functional normal and cervical cancer models.

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Conflict of interest statement

The authors reported no conflict of interests.

Figures

Fig.1
Fig.1
The 3-D in vitro model of the cervix.
Fig.2
Fig.2
The 3-D in vitro models of normal cervix (magnification 400×).
Fig.3
Fig.3
The C33A 3-D in vitro model of cervical cancer at magnification of 200×.
Fig.4
Fig.4
Immunohistochemical staining of Mad 1 from C33A 3-D in vitro model of cervical cancer.
See this image and copyright information in PMC

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References

    1. Kim JB. Three-dimensional tissue culture models in cancer biology[J]. Semin Cancer Biol, 2005, 15(5): 365–377. - PubMed
    1. Ellingsen C, Natvig I, Gaustad JV, et al. Human cervical carcinoma xenograft models for studies of the physiological microenvironment of tumors[J]. J Cancer Res ClinOncol, 2009, 135(9): 1177–1184. - PubMed
    1. Padrón JM, van der Wilt CL, Smid K, et al. The multilayered postconfluent cell culture as a model for drug screening[J]. Crit Rev Oncol Hematol, 2000, 36(2-3): 141–157. - PubMed
    1. Sun T, Jackson S, Haycock JW, et al. Culture of skin cells in 3D rather than 2D improves their ability to survive exposure to cytotoxic agents[J]. J Biotechnol, 2006, 122(3): 372–381. - PubMed
    1. Benam KH, Dauth S, Hassell B, et al. Engineered in vitro disease models[J]. Annu Rev Pathol, 2015, 10(10): 195–262. - PubMed

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