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. 2017:1650:167-176.
doi: 10.1007/978-1-4939-7216-6_10.

In Ovo Electroporation Methods in Chick Embryos

Hidekiyo Harada  1   2 , Minoru Omi  3 , Harukazu Nakamura  4
Affiliations

In Ovo Electroporation Methods in Chick Embryos

Hidekiyo Harada et al. Methods Mol Biol. 2017.

Abstract

To elucidate a gene function, in vivo analysis is indispensable. We can carry out gain and loss of function experiment of a gene of interest by electroporation in ovo and ex ovo culture system on early-stage and advanced-stage chick embryos, respectively. In this section, we introduce in/ex ovo electroporation methods for the development of the chick central nervous system and visual system investigation.

Keywords: Central nervous system; Ex ovo electroporation chick; In ovo electroporation; Long-term misexpression; Tol2 transposon; Transient misexpression; Visual system.

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