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. 2017 Nov;31(11):2532-2535.
doi: 10.1038/leu.2017.260. Epub 2017 Aug 16.

Monitoring molecular response in adult T-cell leukemia by high-throughput sequencing analysis of HTLV-1 clonality

M Artesi  1   2 , A Marçais  3 , K Durkin  1   2 , N Rosewick  1   2 , V Hahaut  1 , F Suarez  3 , A Trinquand  4 , L Lhermitte  4 , V Asnafi  4 , V Avettand-Fenoel  5 , A Burny  2 , M Georges  1 , O Hermine  3   6 , A Van den Broeke  1   2
Affiliations

Monitoring molecular response in adult T-cell leukemia by high-throughput sequencing analysis of HTLV-1 clonality

M Artesi et al. Leukemia. 2017 Nov.
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Longitudinal monitoring of the dominant malignant clone and clone frequency distribution in five ATL patients. (a–e) Evolution of the abundance of the dominant clone relative to all infected cells is represented by longitudinal charts with colored dots corresponding to each time point (diagnosis, relapse, complete remission CR1, CR2 and CR3). Pink area with red dots indicates the period of complete clinical remission (Supplementary Table 1). Samples with a clonally rearranged TCR-γ gene have dots marked with a circle (TCR+). Clone frequency distribution is illustrated by pie charts, each slice representing an independent integration site and its corresponding clonal abundance. The dominant clone (relative abundance per 100 proviral copies, indicated below the pie chart) is depicted in red except for ATL11-Relapse-LN (turquoise, clone switch) and ATL60 (four equally frequent proviruses in a single malignant clone, single TCR-γ rearrangement, Table 1). The remaining underlying clones are shown in gray. PVL: proviral load (tax copies per 100 peripheral blood mononuclear cells (PBMCs)). Absolute abundance (percentage of HTLV-1 insertion sites in PBMCs) was calculated from PVL and the clone’s relative abundance. Absolute abundance of malignant integration sites at CR ATL14-CR1<0.007% (d, PVL: 0.016%, relative abundance within HTLV-1-infected PBMCs, 43%).
See this image and copyright information in PMC

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