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Comment
. 2017 Aug 18;85(9):e00269-17.
doi: 10.1128/IAI.00269-17. Print 2017 Sep.

Suspected Mycoplasma Contamination in the Study "Toll-Like Receptor 2 Recognizes Orientia tsutsugamushi and Increases Susceptibility to Murine Experimental Scrub Typhus"

Wiwit Tantibhedhyangkul  1 , Naharuthai Inthasin  2 , Patimaporn Wongprompitak  2 , Pattama Ekpo  2
Affiliations
Comment

Suspected Mycoplasma Contamination in the Study "Toll-Like Receptor 2 Recognizes Orientia tsutsugamushi and Increases Susceptibility to Murine Experimental Scrub Typhus"

Wiwit Tantibhedhyangkul et al. Infect Immun. .
No abstract available

Keywords: Mycoplasma; Orientia tsutsugamushi; Toll-like receptor 2.

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Figures

FIG 1
FIG 1
O. tsutsugamushi (OT) Karp organisms with or without Mycoplasma orale (Myco) contamination were used to infect HEK-Blue cells (InvivoGen, San Diego, CA), which stably coexpress a human TLR (hTLR) gene and an NF-κB-inducible secreted embryonic alkaline phosphatase reporter gene. Alkaline phosphatase levels in supernatants were measured by spectrophotometry, normalized to those of unstimulated cells, and correlated with fold changes in NF-κB activation. Data represent the mean ± the standard error of the mean of triplicate values and are representative of three independent experiments. Heat-killed Listeria monocytogenes (HKLM) is a positive control for TLR2 activation. To calculate multiplicities of infection (MOIs), O. tsutsugamushi was quantified by real-time PCR. LPS, lipopolysaccharide.
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