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. 2011 Aug 12;4(8):1417-1425.
doi: 10.3390/ma4081417.

Preparation of Chitin Nanofibers from Mushrooms

Shinsuke Ifuku  1 , Ryoki Nomura  2 , Minoru Morimoto  3 , Hiroyuki Saimoto  4
Affiliations

Preparation of Chitin Nanofibers from Mushrooms

Shinsuke Ifuku et al. Materials (Basel). .

Abstract

Chitin nanofibers were isolated from the cell walls of five different types of mushrooms by the removal of glucans, minerals, and proteins, followed by a simple grinding treatment under acidic conditions. The Chitin nanofibers thus obtained have a uniform structure and a long fiber length. The width of the nanofibers depended on the type of mushrooms and varied in the range 20 to 28 nm. The Chitin nanofibers were characterized by elemental analyses, FT-IR spectra, and X-ray diffraction profiles. The results showed that the α-chitin crystal structure was maintained and glucans remained on the nanofiber surface.

Keywords: chitin; mushrooms; nanofibers.

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Figures

Figure 1
Figure 1
Schematic presentation of the cell-wall structure of a typical mushroom.
Figure 2
Figure 2
Preparation procedure of chitin nanofibers from mushrooms.
Figure 3
Figure 3
FE-SEM micrographs of chitin nanofibers prepared from (a) Pleurotus eryngii (b) Agaricus bisporus (c) Lentinula edodes (d) Grifola frondosa (e) Hypsizygus marmoreus. The scale bars are 200 nm in length.
Figure 4
Figure 4
FT-IR spectra of (a) commercially available chitin, and chitin nanofibers prepared from (b) Lentinula edodes (c) Pleurotus eryngii (d) Hypsizygus marmoreus (e) Grifola frondosa and (f) Agaricus bisporus.
Figure 5
Figure 5
X-ray diffraction profiles of (a) commercially available chitin, and chitin nanofibers prepared from (b) Pleurotus eryngii (c) Agaricus bisporus (d) Lentinula edodes (e) Grifola frondosa (f) Hypsizygus marmoreus and (g) commercially available cellulose.
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