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. 1987 Apr;326(6112):471-5.
doi: 10.1038/326471a0.

The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro

G Prelich, M Kostura, D R Marshak, M B Mathews, B Stillman

The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro

G Prelich et al. Nature. 1987 Apr.

Abstract

Cell-free extracts prepared from human 293 cells, supplemented with purified SV40 large-T antigen, support replication of plasmids containing the SV40 origin of DNA replication. A cellular protein (Mr approximately 36,000) that is required for efficient SV40 DNA synthesis in vitro has been purified from these extracts. This protein is recognized by human autoantibodies and is identified as the cell-cycle regulated protein known as proliferating cell nuclear antigen (PCNA) or cyclin.

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