Mechanisms of oxidative stress effects of the NADPH oxidase-ROS-NF-κB transduction pathway and VPO1 on patients with chronic obstructive pulmonary disease combined with pulmonary hypertension

Abstract

Objective: This study explored the oxidative stress effects of the NADPH oxidase-ROS-NF-κB transduction pathway and vascular peroxidase 1 (VPO1) on patients with chronic obstructive pulmonary disease (COPD) combined with pulmonary hypertension (PH).

Patients and methods: 30 patients with pure COPD and 30 patients with stable COPD combined with PH, who were admitted to our hospital from January 2015 to December 2015, were enrolled as the control group and the observation group, respectively. NADPH oxidase activity in mononuclear cells, reactive oxygen species (ROS), NF-κB, VPO1 levels in serum, and further oxidative stress-related indices were compared and analyzed.

Results: Systolic pulmonary pressure (SPAP) (63.65±9.47 mmHg) of the observation group was higher than SPAP (53.13±7.52 mmHg) of the control group (p<0.05); malondialdehyde (MDA) (7.81±2.24 nmol/l) of the observation group was lower than MDA (9.54±3.38 nmol/l) of the control group (p<0.05); superoxide dismutase (SOD) (406.73±147.35 U/ml) of the observation group was higher than SOD (295.16±106.73 U/ml) of the control group (p<0.05). NADPH oxidase activity, ROS and NF-κB levels of the observation group were higher than those of the control group (p<0.05). The average level of serum VPO1 in the observation group (236.71±42.35) ng/L was significantly higher than that of the control group (122.56±34.62) ng/L (p<0.05). In the observation group, the level of SPAP was positively correlated with the concentration of VPO1 (r=0.615, p<0.05), the serum levels VPO1 were negatively correlated with MDA levels (r=-0.537, p<0.05), MDA and ROS levels were negatively correlated (r=-0.482, p<0.05), and the levels of SOD were positively correlated with NF-κB (r=0.427, p<0.05).

Conclusions: The NADPH oxidase-ROS-NF-κB transduction pathway, VPO1, oxidative stress and their complicated interactions are implicated in the occurrence and development of COPD combined with PH.