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. 1987 May;55(5):1321-3.
doi: 10.1128/iai.55.5.1321-1323.1987.

Expression of the S-1 catalytic subunit of pertussis toxin in Escherichia coli

J T Barbieri, R Rappuoli, R J Collier

Expression of the S-1 catalytic subunit of pertussis toxin in Escherichia coli

J T Barbieri et al. Infect Immun. 1987 May.

Abstract

The S-1 subunit of pertussis toxin was expressed as a fusion protein in a strain of Escherichia coli deficient in protein degradation. The fusion protein reacted with anti-pertussis toxin antibody, and, like authentic pertussis toxin, it ADP-ribosylated a 41,000-molecular-weight membrane protein from human erythrocytes.

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