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. 2017 Oct;19(3):476-481.
doi: 10.22074/cellj.2017.3866. Epub 2017 Aug 19.

Effect of Fetal Mouse Lung Tissue Co-Culture on In Vitro Maturation of Mouse Immature Oocytes

Masomeh Belbasi  1 , Seyed Gholam Ali Jorsaraei  2 , Maryam Gholamitabar Tabari  3 , Ramzan Khanbabaei  1
Affiliations

Effect of Fetal Mouse Lung Tissue Co-Culture on In Vitro Maturation of Mouse Immature Oocytes

Masomeh Belbasi et al. Cell J. 2017 Oct.

Abstract

Objectives: The aim of this study was to evaluate the fetal mouse lung tissue co-culture on in vitro maturation (IVM) of mouse immature oocytes.

Materials and methods: In this experimental study, germinal vesicle (GV) oocytes from ovaries of a group of 25 female mice, 6-8 weeks of age, were dissected after being stimulated by 7.5 IU pregnant mare serum gonadotropin (PMSG) through an intraperitoneal (IP) injection. The fetal lung tissues were then prepared and cultured individually. A total number of 300 oocytes were cultured in the following three groups for 24 hours: control group (n=100) containing only base medium, group I (n=100) containing base medium co-cultured with 11.5- to 12.5-day old fetal mouse lung tissues, and group II (n=100) containing base medium co-cultured with 12.5- to 13.5-day old fetal mouse lung tissues. The proportion of GV and metaphase І (MI) oocytes matured into MІІ oocytes were compared among the three groups using analysis of variance (ANOVA). Correlation test were also used to evaluate the successful rate of IVM oocytes.

Results: The proportions of GV oocytes reaching MІІ stage were 46, 65, and 56%, in control, I and II groups, respectively (P<0.05). The percentage of the oocytes remaining at the GV stage were higher in control group as compared with two treatment groups (P<0.05).

Conclusions: This study indicated that fetal mouse lung tissue co-culture method increased the percentage of GV oocytes reaching MII stage.

Keywords: Co-Culture; In Vitro Maturation; Lung Tissue.

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Conflict of interest statement

There is no conflict of interest in this study.

Figures

Fig.1
Fig.1
In vitro maturation (IVM) of immature oocytes and fetal mouse lung tissue co culture. A. Mouse embryos in the uterine horn oocytes at germinal vesicle (GV) stage, B. Immature oocytes before being co-cultured with the fetal mouse lung tissue, C. Immature oocytes with the fetal mouse lung tissue co-culture, and D. Oocytes at metaphase II (MII) stage.
See this image and copyright information in PMC

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