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Comment
. 2017 Aug 24;18(1):159.
doi: 10.1186/s13059-017-1300-5.

New skin for the old RNA-Seq ceremony: the age of single-cell multi-omics

Maayan Baron  1 , Itai Yanai  2
Affiliations
Comment

New skin for the old RNA-Seq ceremony: the age of single-cell multi-omics

Maayan Baron et al. Genome Biol. .

Abstract

New methods for simultaneously quantifying protein and gene expression at the single-cell level have the power to identify cell types and to classify cell populations.

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Conflict of interest statement

Competing interests

The authors declare that they have no competing interests.

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Figures

Fig. 1
Fig. 1
New methods for single-cell protein profiling. In the antibody barcoding with photocleavable DNA platform (ABCD) platform, cells are permeabilized and stained using a panel of antibodies. The tagged DNA is cleaved, amplified by PCR, and sequenced using Nanostring technology. The Abseq method is performed by encapsulating stained cells, tagging each cell with a unique barcode, and PCR amplifying and sequencing using Illumina technology. The cellular indexing of transcriptomes and epitopes by sequencing (CITE-Seq) method utilizes poly(A) oligonucleotides to profile cell-surface proteins and it can be coupled with single-cell RNA-seq protocols such as Drop-Seq and Chromium (10×)
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Comment on

References

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