Generation and Analysis of Chromosomal Contact Maps of Bacteria

Abstract

This methods article described a protocol aiming at generating chromosome contact maps of bacterial species using a genome-wide derivative of the chromosome conformation capture (3C) technique. The approach is readily applicable on a broad variety of gram + and gram-bacterial species. It describes and addresses known caveats and technicalities associated with the technique, and should be of interest to any laboratory interested to perform a multiscale analysis of the genome structure of its species of interest.

Keywords: 3C; Chromosome conformation capture, Hi-C; Genome organization; Nucleoid.