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Comparative Study
. 2018 Jan-Mar;49(1):120-127.
doi: 10.1016/j.bjm.2017.05.003. Epub 2017 Aug 6.

A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG

Jean Claude Assaf  1 , Ali Atoui  2 , André El Khoury  3 , Ali Chokr  4 , Nicolas Louka  3
Affiliations
Comparative Study

A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG

Jean Claude Assaf et al. Braz J Microbiol. 2018 Jan-Mar.

Abstract

Several strains of lactic acid bacteria (LAB), frequently used in food fermentation and preservation, have been reported to bind different types of toxins in liquid media. This study was carried out to investigate the effect of different concentrations of Lactobacillus rhamnosus GG (ATCC 53103) to bind aflatoxin M1 (AFM1) in liquid media. AFM1 binding was tested following repetitive washes or filtration procedures in combination with additional treatments such as heating, pipetting, and centrifugation. The mixture of L. rhamnosus GG and AFM1 was incubated for 18h at 37°C and the binding efficiency was determined by quantifying the unbound AFM1 using HPLC. The stability of the complexes viable bacteria-AFM1 and heat treated bacteria-AFM1 was tested. Depending on the bacterial concentration and procedure used, the percentages of bound AFM1 by L. rhamnosus GG varied from as low as undetectable to as high as 63%. The highest reduction in the level of unbound AFM1 was recorded for the five washes procedure that involved heating and pipetting. Results also showed that binding was partially reversible and AFM1 was released after repeated washes. These findings highlight the effect of different treatments on the binding of AFM1 to L. rhamnosus GG in liquid matrix.

Keywords: Aflatoxin M1; L. rhamnosus GG; Lactic acid bacteria; Milk.

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Figures

Fig. 1
Fig. 1
Effect of washes on L. rhamnosus GG/AFM1 complex stability. Binding was determined after viable and heat treated bacteria (1010 CFU) were incubated with AFM1 (1 mL, 50 μg/L) at 37 °C for 18 h with previous pipetting. The complexes formed were exposed to five washes with 1 mL of PBS and the residual AFM1 was quantified. Error bars represent the SD (standard deviation).
Fig. 2
Fig. 2
Effect of washes on L. rhamnosus GG/AFM1 complex stability. Binding was determined after viable and heat treated bacteria (1010 CFU) were incubated with AFM1 (1 mL, 50 μg/L) at 37 °C for 18 h without previous pipetting. The complexes formed were exposed to five washes with 1 mL of PBS and the residual AFM1 was quantified. Error bars represent the SD.
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